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1、临床医学论文・VEGF在大鼠缺血再灌注损伤肝组织中的表达及其意义【摘要】FI的：探讨血管内皮生长因子(vnsculdTendothelialgrowthfactor,WF)在缺血再灌注损伤肝组织屮的表达及意义。方法：利用雄性SD人鼠建立部分肝血流阻断动物模型，肝血流阻断90min后恢复血流灌注，再灌注后1,6h处死大鼠收集标本。分别采用全口动生化分析仪、HE染色，比色法、ELISA法测定血清转氨酶(ALT,AST),肝组织病理改变,肝组织髓过氧化物酶(MP0)的活性以及VEGF蛋白的含量。结果：与假手术
2、组相比，缺血再灌注组(I/R组)缺血肝脏MP0活性，肝组织内VEGF蛋白的含量以及ALT,AST在再灌注1h即升高，且缺血再灌注6h时较1h明显升高；缺血肝脏病理切片可见大量炎症细胞浸润，6h炎症反应更为剧烈，部分肝细胞呈点状坏死。结论：VEGF蛋白表达的上调可能参与了大鼠肝脏缺血再灌注过程屮肝脏的损伤。【关键词】肝脏；缺血；再灌注损伤；血管内皮生长因子[Abstract]Objcctivc:Toinvestigatetheexpressionofvascularendothelialgrowthfac
3、tor(VEGF)intheliverischemia/reperfusioninjuryandsignificancc.Mcthods：ThemaleSDratswereusedtocstablishpartialhepaticvascularexclusionanimalmode1.Afterischemiafor90min,SDratsliverwerereperfused,andthenliverspecimenswerecollectedat1hand6hafterreperfusionres
4、pectively.Routineassayswereperformedfortestingthelevelsofalanineaminotransferase(ALT),aspartateaminotransferase(AST)andtheactivityofhepaticmye1operoxidase(MPO)・ThechangesofTEGFproteininischemicliverlobesweredeterminedbyELISA・Thelivertissuespathologicalc
5、hangeswereevaluatedinhematoxylinandcosin(HE)stained・Results：After90minofpartial1iverischemia,theAST,ALTlevelinI/Rgroupwassignificantlyincreasedascomparedwiththesham・opcratcd(SH)group,andAST,ALTlevelatthe6hreperfusionwasmarklyhigherthanthatatthe1hreperfus
6、ion.TheexpressionofVEGFproteinandtheMPOactivityinhepatictissueswereremarkablyup-regulatedinI/RgroupthanintheSHgroupalthelimepointof1hand6hreperfusion.Theywerehigheral6hreperfusionthanthatat1h.ForHEstaining,theinfiltrationofmanyinflammatorycel1swasobserve
7、dinlivertissueandbecamemuchsevererat6hposT・TcpcTfusionlivertissue.Conclusion：Theup・regulationofVEGFproteininhepaticI/Rinjuryprocessmightbeinvolvedintheprocessofliverinjury.[Keywords]liver；ischemia；reperfusioninjury；vascularendothelialgrowthfactor缺血再灌注损伤是
8、肝移植、复杂肝切除术屮常常涉及的共同病理过程，多种细胞因子参与并介导了肝缺血再灌注损伤过程屮的各个环节。近年来，国外许多学者研究发现血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)与缺血再灌注损伤密切相关[1,2]。血管内皮生长因子又称血管通透因子(vascularpermeabi1ityfactor,VPF),它是一种二聚糖,蛋白相对分子质量为34〜45kD,由两个相同的亚基组成，是内皮细胞产生
