Notch家族蛋白、LCHAD在早发型重度子痫前期患者胎盘组织中的表达及意义.pdf

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1、临京医学工程2014年6月第21卷第6期·699··论著·(实验研究)Notch家族蛋白、LCHAD在早发型重度子痫前期患者胎盘组织中的表达及意义赵莉娜，马瑞霞，刘国成(广东省妇幼保健院，广东广州510010)【摘要】目的探讨Notch家族蛋白、长链三羟基酰基辅酶A脱氢酶(LCHAD)的表达与早发型重度子痫前期发病的关系，揭示早发型重度子痫前期发病机理方法选择早发型重度子痫前期患者45例、晚发型重度子痫前期患者42例为研究组，40例正常分娩者为对照组．免疫组化法检测Notch家族蛋白、LCHAD在胎盘绒毛组织中的分布和表达水平。结果各组胎盘组织的合体滋养细

2、胞、血管内皮细胞、蜕膜细胞中均可以检测到Notch家族蛋白、LCHAD的表达。早发型重度子痈前期组Notch家族蛋白、LCHAD表达均显著低于晚发型重度子痫前期组及对照组。各组胎盘组织中Notch家族蛋白表达与LCHAD表达水平呈正相关。结论早发型重度子痫前期组胎盘中Notch家族蛋白、LCHAD低表达与疾病的发病发展密切相关。二者通过影响滋养细胞浸润．胎盘浅着床在早发型重度子痫前期发生发展中起重要作用。【关键词】早发型重度子痫前期；Notch家族蛋白；长链三羟基酰基辅酶A脱氢酶中图分类号：R714．245文献标识码：Adoi：10．39690．issn．

3、1674—4659．2014．06．0699ExpressionLevelsandC。linicalValuesofNotchProteinsandLCHADinthePlacentaofEarly—onsetSeverePreeclampsiaZHA0Lina,MARuixia,LIUGuochengrGuangdongWomenandChildrenHospital,Guangzhou510010,China)【Abstract】ObjectiveTostudytherelationshipbetweenNotchproteinsandLCHADand

4、early—onsetseverepreeclampsia，andtorevealthepathogenesisofthedisease．Methods45early—onsetseverepreeclampsiaand42late—onsetpatientswereselectedasastudygroup，and40patientswithnormaldeliverieswereselectedasacontrolgroup．WedetectedthedistributionandexpressionlevelsoftheNotchproteinsan

5、dLCHADinplacentalvilluswithanimmunohistochemicalmethod．ResultsTheNotchproteinsandLCHADcouldbedetectedinsyncytiotrophoblasts，vascularendothelialcellsanddecidualcellsoftheplacenta．TheexpressionlevelsoftheNotchproteinsandLCHADintheearly-onsetpatientsweresignificantlylowerthanthoseint

6、helate—onsetpatientsandthoseinthecontrolgroup．TheexpressionlevelsofNotchproteinshadapositivecorrelationwiththoseofLCHAD．ConclusionsTheearly—onsetseverepreeclampsiaiscloselyrelatedtothelowexpressionlevelsofNotchproteinsandLCHAD，whichplayanimportantroleduringtheshallowimplantationof

7、theplacentabyinfluencingtheinfiltrationoftrophocytesandtheonsetandprogressionofearly—onsetseverepreeclampsia．【Keywords】Earlyonsetseverepreeclampsia；Notchfamilyproteins；Long—chain3hyroxyacylacyl—COAdehydrogenase(LCHAD)早发型重度子痫前期患者发病早、病情重，比晚发型者有1．2试验方法着严重的围产和产科结局，其发病机制至今不清，目前滋养细1．2．1

8、标本制备无菌剪取胎盘脐带根部附着区母体面中央1．5胞浅着床学说成为