阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及Bcl-2和Bax表达的影响.pdf

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1、2015，Vo1．32，No．3药物研究阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及Bc1．2和Bax表达的影响汝文文，和娴娴，钤莉妍，孙阳恩，韩丽，王东亮(山东东阿阿胶股份有限公司，国家胶类中药工程技术研究中心，山东聊城252201)[摘要]目的：探讨阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及其相关基因Bcl-2和Bax表达的影响。方法：选用4月龄雌性大鼠为正常对照组，14月龄阴道细胞学表现为动情期延长的雌性大鼠为衰老大鼠模型。模型动物随机分别给予阿胶或戊酸雌二醇片治疗。采用原位末端核苷酸标记法(TUNEL)检测卵巢细胞凋亡，免疫组化法检测细胞凋亡相关因子Bel-2，Bax。结果：阿胶高、中

2、、低剂量组卵巢细胞凋亡阳性表达与模型组比较有显著性差异，同时可使卵巢凋亡基因Blc-2表达增强，促凋亡基因Bax表达减弱，Bel-2／Bax比例增加。结论：阿胶通过抑制Bax蛋白的表达，上调Bcl-2蛋白表达，抑制卵巢颗粒细胞凋亡，进而改善卵巢功能。[关键词]阿胶；围绝经期大鼠；细胞凋亡；Bcl-2；Bax[中图分类号]R282[文献标识码]A[文章编号]2095—3593(2015)03—0147—04EfectsofCo／／aCoriiAsinionOvarianGranulosaCellsApoptosisandExpressionofBcl-2andBaxinPerime

3、nopausalRatsRUWen—wen，HEXian—xian，QIANLi—yan，SUNYang—en，HANLi，WANGDong—liang(ShandongDong—EE-JiaoCo．，Ltd．，NationalEngineeringTechnologyResearchCenterofRubberMedicine，ShandongLiaocheng252201，China)[Abstract]Objective：TostudytheeffectsofCollaConiAsinionovariangranulosacellsapoptosisandexpressio

4、nofBc1-2andBaxinpefimenopausalrats．Methods：SDfemaleratsof4monthsoldweresettobethenormalcontrolgroupandSDfemaleratsof14monthsoldwhoseoestruselongationincolpocytologyappearanceweresetasthesenilefemaleratmode1．Theseratsweredividedinto5groupsrandomly，asfollows：highdoseCollaCoriiAsinigroup，middled

5、oseCoUaCoriiAsinigroup，lowdoseCollaConiAsinigroup，estradiolvalerategroup，modelgroup．After8weeksintheinterventionperiod，ovarianapoptosisandtheexpressionofovariangranulosacellBcl-2．BaxweremeasuredbyTUNELandimmunohisto-chemistrymethods．Results：Highandmiddledosec0ffnConiAsinigroupscouldde-creasea

6、poptosisinovariangranulosecells(P<0．05)，andcouldimprovetheexpressionofBcl-2intheovariesandweakenedtheex—pressionofBax，whichthenincreasetheratioofBcl-2／Bax．Conclusion：CollaCoriiAsinicoulddelayovariangranuloseceilsapoptosisthroughinhibitingtheexpressionofBax，increasingtheexpressionofBel-2during

7、perimenopausalperiod，whichmightbeonereasonofCollaCoriiAsinipostponedtheovarianfailureandcuredperimenopausalsyndrome．[KeyWords]CollaCodiAsini；Perimenopausalrats；Cellsapoptosis；Bcl一2；Bax围绝经期综合征(perimenopausalayndrome，PMS)骨质疏松的一组征候群。阿胶为马科动物驴(“是指