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时间:2020-05-24
《气腹对大鼠肝内胆管上皮细胞凋亡和Bcl-2/Bax基因表达的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、临床麻醉学杂志2013年n月第29卷第ll期JClinAnesthesiol,November2013,Vo1.29,No.1l.实验研究.气腹对大鼠肝内胆管上皮细胞凋亡和Bcl一2/Bax基因表达的影响李文成王刚陈解元邓龙姣【摘要】目的探讨气腹所致缺血一再灌注损伤对大鼠肝内胆管上皮细胞凋亡和Bcl-2/Bax基因表达的影响。方法SD大鼠4O只,随机均分为五组,其中S组为假手术组,经脐插入气腹针但不充气;P1和P2组气腹压力为10ITlrnHg,P3和P4组气腹压力为15mmHg,Pl和P3组气腹时间为2h,P2和P4组气腹时间为4h。各组大鼠达到预定
2、气腹时间排气后6h取血清测定肝功能;取左肝内叶肝组织,TUNEL染色测定肝内胆管上皮细胞的凋亡指数(AI),免疫组化检测肝内胆管上皮细胞内Bcl一2和Bax基因表达评分(IHS评分)。结果S组大鼠血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)水平明显高于其它四组(P3、O.05)。结论长时间气腹可引发肝内胆管上皮细胞内的Bax基因高表达,使得Bcl-2/Bax表达水平发生变化,导致肝内胆管上皮细胞凋亡增加。【关键词】气腹;胆管上皮细胞;凋亡;Bcl一2/Bax基因EffectofpneumoperitoneumonapoptosisandBcl-2/BaxexpressioninintrahepaticbileductepithelialcellsinratsLIWen—cheng,WANGGang,CHENe-yuan,DENGLong-jiao.DepartmentAnesthesiology,Guangdong4、GeneralHospitalGuangdongAcademyofMedicalSciences,Guangzhou510080。inaCorrespondingauthor:WANGGang,Email:wggdry@sina.corn[Abstract]ObjectiveToinvestigatetheeffectsofcarbondioxidepneumoperitoneumonapoptosisandBcl一2/Baxexpressioninintrahepaticbileductepithelialcellsintherats.Methods5、FortyhealthymaleSDratswererandomlydividedintofivegroups(一8foreachgroup)andsubjectedtothedifferentinsuflationpressuresorpneumoperitoneumtimeduration:nogascontrol(groupS),10mrnHg-2hours(groupP1),10mmHg-4hours(groupP2),15rnrnHg-2hours(groupP3)and15mInHg-4hours(groupP4).Liverfunctio6、nwasevaluatedusinganautomaticbiochemicalanalyzer.ApoptoticindexesofintrahepaticbileductepithelialcellsweremeasuredbyTUNELin40biliaryspecimens,andtheexpressionofBcl一2/Baxproteinsinintrahepaticbileductepithelialcellsweredeterminedbyimmunohistochemistry。respectively.ResultsASTandAL7、Tingroupspl,P2,P3andP4weresignificantlydisturbedcomparedtothoseingroupS(P<0.01).ALPandTBAingroupsP2andP4weresignificantlyhigherthanthoseinothergroups(P<0.05).ApoptoticindexofintrahepaticbileductepithelialcellsgroupsP2andP4weresignificantlyhigherthantheotherthreegroups(P8、erewerenodifferencesintheexpressionofBcl-2prote
3、O.05)。结论长时间气腹可引发肝内胆管上皮细胞内的Bax基因高表达,使得Bcl-2/Bax表达水平发生变化,导致肝内胆管上皮细胞凋亡增加。【关键词】气腹;胆管上皮细胞;凋亡;Bcl一2/Bax基因EffectofpneumoperitoneumonapoptosisandBcl-2/BaxexpressioninintrahepaticbileductepithelialcellsinratsLIWen—cheng,WANGGang,CHENe-yuan,DENGLong-jiao.DepartmentAnesthesiology,Guangdong
4、GeneralHospitalGuangdongAcademyofMedicalSciences,Guangzhou510080。inaCorrespondingauthor:WANGGang,Email:wggdry@sina.corn[Abstract]ObjectiveToinvestigatetheeffectsofcarbondioxidepneumoperitoneumonapoptosisandBcl一2/Baxexpressioninintrahepaticbileductepithelialcellsintherats.Methods
5、FortyhealthymaleSDratswererandomlydividedintofivegroups(一8foreachgroup)andsubjectedtothedifferentinsuflationpressuresorpneumoperitoneumtimeduration:nogascontrol(groupS),10mrnHg-2hours(groupP1),10mmHg-4hours(groupP2),15rnrnHg-2hours(groupP3)and15mInHg-4hours(groupP4).Liverfunctio
6、nwasevaluatedusinganautomaticbiochemicalanalyzer.ApoptoticindexesofintrahepaticbileductepithelialcellsweremeasuredbyTUNELin40biliaryspecimens,andtheexpressionofBcl一2/Baxproteinsinintrahepaticbileductepithelialcellsweredeterminedbyimmunohistochemistry。respectively.ResultsASTandAL
7、Tingroupspl,P2,P3andP4weresignificantlydisturbedcomparedtothoseingroupS(P<0.01).ALPandTBAingroupsP2andP4weresignificantlyhigherthanthoseinothergroups(P<0.05).ApoptoticindexofintrahepaticbileductepithelialcellsgroupsP2andP4weresignificantlyhigherthantheotherthreegroups(P8、erewerenodifferencesintheexpressionofBcl-2prote
8、erewerenodifferencesintheexpressionofBcl-2prote
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