脑组织中的海马取法

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1、..取活组织需要玻璃分针，止血钳和冰袋，组织剪。取的时候先断头，从枕骨大孔往前剪开颅骨，完全暴露脑组织，用玻璃分针别离脑组织和颅骨。别离下来的全脑组织置于冰袋上，用玻璃分针从后往上揎起端脑，即可看见海马，左右各一，之间有组织相连，别离的时候要轻柔，尽量防止破坏组织构造。别离下来的组织根据试验安排做后续处理.step1:Thisisadorsalviewofamouse’sskull,inordertoaccessthebrainyoucancutalongthecoronalsutureandsagittalsuturethenpulloffbothsidesof

2、parietalboneandinterparietalbone.这是大鼠头部反面观，为了接触到脑，需沿冠状缝和矢状缝切开，然后把顶骨和枕间骨取下。..word.zl.....word.zl...step2:Nowtheskullisopenandyoucanseethebrainclearly,inordertogetthehippocampusyou’dbetterkeepthebraininthecranialcavitysothebrainwillnotmovewhenyoucontinuewiththefollowingsteps现在头盖骨被翻开，你可以

3、清楚地看到大脑，但为了获得海马你最好把大脑保持在颅腔，这样在你进展以下操作时大脑就不会移动。step3:Inordertoexposethehippocampusyouneedtoremovethecerebralcortexcoveringit.Thefirstincisionisattheendofthehemisphere;theincisionshouldbeabout0.7mmdeepformostadultmousethatyoumightnothurtthehippocampuswhiletoexposeit.The2ndincisionisabou

4、t1.5-2mminfrontofthefirstone,thisincisionyouneedcutintothelateralventricle,bothoftheincisionsgototheventralofthebrainandmeetthere.Nowthispieceofcortexisfree,pull..word.zl...itup,youwillseethehippocampusjustlikeinthispicture,alsoyoucanseetheCSFintheopenedventricle为了看到海马你需要移动覆于其上的大脑皮层。第

5、一个切口在半球的末端，对大多数成年大鼠来说，此切口应约0.7mm深，这样才能保证不伤害到海马。第二个切口在第一个切口前方约1.5-2mm处，此切口需要切入到侧脑室，两个切口在大脑腹侧衔接。现在这块大脑皮层就是游离的了，把它取下来，你就可以看到如以下图的海马，你也可以看到翻开的脑室的CSF〔脑脊液〕。step4:Keepworkingontheothersideofthebrainpullupbothsidesofthecortexthatcoveringthehippocampusalongtheventricle.Nowyou..word.zl...cansee

6、thedorsalpartofthehippocampus.Separatetherestofthehippocampusfromthecortexcoveringitalongthesurfaceofthehippocampustowardstheventralpartofthehippocampus继续沿脑室把覆盖在海马上面的两边皮层揭下。现在你能看到海马的背部。把余下的局部沿着海马的外表直到海马的腹部局部从覆与其上的大脑皮层中别离出来.step5:Keepworkingontheothersideofthebrainpullupbothsidesofthec

7、ortexthatcoveringthehippocampusalongtheventricle.Nowyoucanseethedorsalpartofthehippocampus.Separatetherestofthehippocampusfromthecortexcoveringitalongthesurfaceofthehippocampustowardstheventralpartofthehippocampus...word.zl...step6:Nowyouneedtofreethehippocampusfromthesurroundingtissu

8、e现在需要