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1、猪冷冻精子的体外受精与单精子胞浆内注射南京农业大:,报2010,33(1):7IIournal1({曲lgAgricuhuraIUnivenit)李阿荣,周冬梅,.冷桁子的外受精Wfl-fi.F~g内注射fJ].南京农业大学,2010,33(1):71—75猪冷冻精子的体外受精与单精子胞浆内注射李宁,芮荣,周冬梅,剧世强,林鹏飞,郝元斌(南京农,人学动物I矢学院,江苏南』210095)摘要:用冷冻精子进行外受精和单精子胞浆内注射(ICSI),并研究添』J口,J一半胱氨酸及不同成熟培养法对猪ICSI胚胎发育的影响结果表fJ
2、J]:J1j猪冷冻精子生产的1CSI胎卯裂率及囊胚发育率分别为68.3%和11.4%,均低丁:用冷冻精厂进体外受精(1V})所拔得的卯裂半和囊胚发育牢(分删为76.9%和19.2%,P<O.05)培养液f{I添加1.71tmm1.,J半胱氰酸培养3h,无论是卯裂率,桑椹胚发育半,还足睫胚发育率,均未得到明显政善(P>O.05);采用含0.4%BSA的NCSU一23培养液进仃猪即母细胞成熟培养,ICSI胚胎卯裂率虽叫显高,但各组所扶胎发育率均无着(/50.05),表ff』】昕川卯母绌胞的成熟力'法并小时ICSI
3、胚胎发育产生明的影响;JI】颗粒细胞或卯细胞培养ICSI胎后,卯裂半l1JJ提高(P<O.05),但各绀暖胚发育率无统计学差异关键词:猪;冷冻精f;体外受精;精了胞浆内注射中图分类号:$828;s814.8文献标志码:A文章编号:10002030(2010)O1007105/nvitrofertilizationandintracytoplasmicsperminjectionusingfrozenboarspermatozoaLINing,RUIRong,ZHOUDong—mei,JUShi—qiang,LINP
4、eng—fei.HAOYuan—bin(CollegeofVete~'inaUMedicine,NanjingAgri(-uhuralUnive~'sih.r,Nanjing210095,Chi㈨)Abstract:Frozel1])oalsperlllaIozoawreusedtoconductinvitrotertilization(IVF)andintraeytoplaslnicsrIllinjecti()nfICSI1inswine.l'hPP(Iofadditim】(Jf,J～('ysteineintomedi
5、umanddiffere~Iteu]luremethodformatufa!i(}nwasals()investigateft._rt】esuhsshowedthatICSIresu]l((1it1igIfifieantlylowe[cleavageratean(1blasto(,vstpercentage(68.3%versus76.9%IIIl(I11.4%VersLIS19.2%,respectively(<O.05)than1]losefromIVFgroup.Additionof1.71mlllO1.LL
6、-cysteinintofne(jiuffJ"j(uJ一[illeICSIPIII})r.vosfor3hi『l1I1r(),edneithercleavagerateaswellasdevelopmentalrateto『I1oru1anorbls[oevsII)er(el1tagPsignificantIv(P>O.05).Using0.4%BSANCSU一23mediumforthematurationofport『le00cvtesseeme(t10viel(1highe11(Ieavagerate()fI
7、CSIembryosthin1othergroups.ttowevet,thedeveloptnentalrateotembryosfl'on1ea(?hgrouphadn0(tifferences(P>O.05).indi(a—tingthatthematul'ationmelhodlnightnotimpactthedevelopmentotICSIemb~wos.Afire(0一(.uIture,itl1l1anuI()sa(.e11s0r(.ul1lu1us(ells,theeleaxagerale()fI
8、(]SIenlI)ryoswasin~[)rovedsignificantlyrthanthatoftlleeOlltl-01(P<O.05),Imttl1PrPisn()statisti(.(1ifleren('einthe})lastoeyst1)P九Plttageaffl()Ilggr()ups.Keyw
