石蜡切片原位杂交免疫组化.doc

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1、病理检验技术江汉大学医学与生命科学学院病理学与病理生理学教研室2004年4月内容提要本书介绍组织病理学常用的多种技术。包括常见的技术，即福尔马林固定、石蜡包埋、HE染色技术，同时也介绍了常用的特殊染色技术，免疫组织化学技术，并简明地介绍了分子生物学技术的基本理论以及在组织病理学中的应用。全书理论与实践并重，经典与改良结合，内容丰富，通俗易懂，实用性强，为实验室工作的必备参考书。适合从事病理学、组织学、生物学、法医学等的科研教学和临床工作的技术人员、教师、医师和研究生使用。同时也适合临床医学检验专业中专、专科生使用以及临床医学专业专升本学生和临床医学专业本科生选用。第一篇石蜡切片技术第一章

2、组织标本的处理第一节取材··············································5第二节组织的固定········································6第三节大体标本的处理和固定·····························14第四节陈列标本的固定···································15第五节脱钙·············································16第六节组织的冲洗······································

3、·18第一章石蜡包埋技术第一节脱水··············································19第二节透明··············································21第三节浸蜡··············································22第四节包埋··············································22第三章切片技术第一节切片刀············································25第二节切片机········

4、····································27第三节石蜡切片的制作····································27第四节特殊组织石蜡切片的制作····························29第五节石蜡切片的异常及处理······························29第四章染色与染色剂第一节染色的基本原理····································31第二节染色剂染色的化学基础······························33第三节染色剂的分类···········

5、···························34第四节常用染色剂及配制··································35第五节苏木素—伊红染色法································39第六节封固剂············································41第五章特殊染色技术第一节结缔组织染色法····································43第二节脂类染色法·······································47第三节糖原及粘液染色法······

6、····························48第四节色素染色法········································50第五节神经组织染色法····································52第六节核酸及核蛋白染色法································52第七节肌肉组织染色法····································53第八节病原微生物染色法··································54第九节特殊染色技术的应用················

7、················57第二篇免疫组织化学和亲和免疫组织化学技术第一章免疫组织化学技术第一节基本原理··········································62第二节染色步骤··········································67第三节常用试剂的配制····································72第二章亲和免疫组织化学技术第一节基本原