纳米雄黄对皮肤鳞癌a431细胞株抑制增殖及抑制新生血管作用的实验研究

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1、提要目的：对皮肤肿瘤进行中医文献复习，并通过检测人皮肤鳞癌A431细胞用纳米雄黄处理后细胞增殖抑制及血管生长调控因子表达情况，探讨纳米雄黄抑制血管生长抗肿瘤的作用机理。方法：查询并复习中医文献资料，对皮肤鳞癌A431细胞进行体外培养，按实验分组加药后观察其形态及生长状况变化，并分别用MTT法、免疫组织化学法和RT-PCR法检测加药后细胞增殖抑制、色素上皮源性因子(PEDF)和VEGFmRNA表达情况，探讨纳米雄黄抑制皮肤鳞癌新生血管生成的作用机理。结果：纳米雄黄可以抑制人皮肤鳞癌A431细胞增殖，且与顺铂联合应用有协同作用，并发现纳米雄黄抑制A431细胞增殖的作用与上调PEDF的表

2、达和下调VEGF的表达以抑制新生血管生成有关。结论：体外实验表明纳米雄黄可显著抑制人皮肤鳞癌A431细胞增殖，并通过抑制其新生血管生成达到抗肿瘤细胞生长的效果，与顺铂有协同作用。关键词纳米雄黄；人皮肤鳞状细胞癌A431细胞；肿瘤血管生成；细胞增殖ExperimentalResearchofRealgarnanoparticlesoncutaneoussquamousCellcarcinomaintheInhibitionofproliferationandNeovascularizationsuppressionSpeciality:ClinicalcombinationMedic

3、ineofTCMWithWesternMedicineAuthor:LinYanyanTutor:ProfessorLiXiurongAbstractObjective:ReviewtheliteratureofTCMonskintumor.DetectthecellproliferationandbloodvessalgrowthregulatoryfactorexpressionofskinsquamouscellcarcinomaA431cellstreatedwithnanometer,thenexploretherealgarnanoparticlestoanti-tum

4、ormechanismofaction.Methods:SkinsquamouscellcarcinomaA431cellswereculturedinvitro.Doseintheexperimentalgroupsandobservethechangesinitsmorphologyandgrowthconditions,respectively,usingtheMTTassay,immunohistochemistryandRT-PCRassaydosinginhibitionofcellproliferation,theexpressionofpigmentepitheli

5、um-derivedfactor(PEDF)andVEGFmRNA.Results:RealgarnanoparticlescaninhibithumanskinsquamouscellscarcinomaA431cellsproliferationandsynergywithcisplatin.Realgarnanoparticlescanru-regulatetheexpressionofPEDFanddowntheexpressionofVEGFtoinhibitangiogenesis.Conclusion:InvitroexperimentsshowedRealgarna

6、noparticlescansignificantlyinhibithumanskinsquamouscellcarcinomaA431cellsproliferationandtreatcancerbyinhibitingangiogenesisgeneratesynergywithcisplatin.KeywordsRealgarnanoparticles;SkinsquamouscellcarcinomaA431cells;inhibitingangiogenesis;Inhibitionofproliferation目录引言.........................

7、......................................................................................................1第一部分实验研究.............................................................................................................3一实验方法和结果.......