针刺对大鼠慢性脊髓损伤hsp70与inos表达的影响

《针刺对大鼠慢性脊髓损伤hsp70与inos表达的影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、汕头大学医学院硕士研究生论文Fortyratswererandomlyallocatedtoamodelgroupincluding30ratsandacontrolgroupincluding10rats(thatis,Agroup).Themodelgroupreceivedmoderatechronicprogressivecompressionwithin8weeks，thenthatwererandomlyallocatedtooneofthreegroupsincluding10ratseach:Bgroupreceivedasustai

2、nedcompression;andCgroupreceiveddecompression;DgroupreceivedEAtreatment.Clinicalneurologicalfunctionwasassessedwithcombinedbehavioralscore(CBS).At11weeksafterinjuried,allanimalsweresacrificed,andhistopathologicalchangeswereobserved,andtheexpressionsofHSP70andiNOSonthesessionoft

3、heratspinalcordwasexaminedwithimmumohistochemicalmethod.Results(1)X-rayrevealedthevertebralcanalencroachmentratewas30%-60%after8weeks.Eachanimalinthemodelgroupdevelopedclinicalsignsofmyelopathy.CBSinCandDgroupsshowedaconsistenttrendtowardimprovedfunction，andthemeanCBSfortheDgro

4、upwassignificantlybetterthanfortheCgroup(P<0.05).(2)HSP70andiNOSimmunohistochemistryshowed，Afterspinalcordinjury,HSP70andiNOSexpressionsincreasedintheneurons.HSP70wasfurtherenhancedandiNOSwasdiminishedintheelectro-acupuncturegroups.Meanwhile，CBSwasimproved,too.Thereweresignific

5、antdifferencesbetweenthegroupsofelectro-acupunctureanddecompression(P<0.05).Conclusions(1)Theanimalmodelinthisstudy,whichaccurately6万方数据汕头大学医学院硕士研究生论文representedthediseaseprocessofchroniccompressivemyelopathy(CCM),isanidealchronicprogressivecompressivespinalcordinjuryanimalmode

6、l.(2)HSP70andiNOSexpressionsincreasedafterChronicspinalcordinjuryanddecreasedafterdecompression.(3)HSP70wasfurtherenhancedresultingfromelectro-acupuncture,whichwasnegativefortheexpressionofiNOS.Itcouldrelievethesecondaryspinalcordinjuryandprotecttheneuronandpromotebehavioralfun

7、ctionrecoveryofexperimentalanimals.(4)WesuggestthatHSP70takepartinthetreatmentofelectro-acupuncture.Electro-acupuncturefacilitatestherepairsofthespinalcordinjuried.Keywordselectro-acupuncture;spinalcordinjury;HeatShockProteins;NitricOxideSynthase7万方数据汕头大学医学院硕士研究生论文前言慢性压迫性脊髓损伤(C

8、hroniccompressivespinalcordinjury，SCI)是中老年的常见多发疾病之一。慢性