高氧液预处理对兔脊髓缺血损伤的保护作用及机制

《高氧液预处理对兔脊髓缺血损伤的保护作用及机制》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、1870第四军医大学学报(JFourthMilMedUniv)2003;24(20)·研究原著·文章编号:100022790(2003)2021870205高氧液预处理对兔脊髓缺血损伤的保护作用及机制1234212桑韩飞,张英民,徐礼鲜,程　虹,巩　固,梅其炳,熊利泽(第四军医大学:1234基础部药理学教研室,西京医院麻醉科,口腔医学院麻醉科,基础部病理学教研室,陕西西安710033)ProtectiveeffectofhyperoxicsolutionD+HgroupandDgroup(P>0.05);T

2、herewasastongcorre2lationbetweentheneurologicfunctionscoresandthenumberofpreconditioningonspinalcordischemicnormalneuronsinanteriorhornofspinalcord(r=0.804,P

3、protectiveeffectonspinalcordischemicinjuryinSANGHan2Fei,ZHANGYing2Min,XULi2Xian,4212CHENGHong,GONGGu,MEIQi2Bing,XIONGLi2Zerabbitsandoxygenfreeradicalsmaybeinvolvedintheprotec214DepartmentofPharmacology,DepartmentofPathology,tiveeffect.2【keywords】freeradica

4、ls;hyperoxicsolution;preconditioning;SchoolofBasicMedicine,DepartmentofAnesthesiology,Xijing3spinalcord;reperfusioninjuryHospital,DepartmentofAnesthesiology,StomatologicalCol2lege,FourthMilitaryMedicalUniversity,Xipan710033,China【摘　要】目的:探讨高氧液预处理对兔脊髓缺血损伤是否有

5、【Abstract】AIM:Toinvestigatewhetherhyperoxicsolutioin保护作用及氧自由基是否参与这种保护作用.方法:32只成preconditioninghasprotectiveeffectonspinalcordischemicin2年雄性新西兰兔随机分成4组(每组8只),即对照组(C组)、juryinrabbitsandwhetheroxygenfreeradicalsareinvolvedin高氧液预处理组(H组)、二甲基硫脲(DMTU,自由基清除theprotec

6、tiveeffect.METHODS:Thirty2twomaleNew剂)+高氧液预处理组(D+H组)及DMTU组(D组).H组Zealandsrabbitswererandomlyassignedto4groups(8in每天静脉给予10mL·kg-1高氧液,20min匀速泵完,连续5eachgroup):Controlgroup(C),hyperoxicsolutionprecondi2d;C组用同样方法给予等容量生理盐水;D+H组在每次高氧tioning(H),dimethylthiourea(DM

7、TU,freeradicalsscavenger)-1液预处理前1h静脉给予500mg·kgDMTU,其他处理同H+hyperoxicsolutionpreconditioninggroup(D+H)andDMTU-1组;D组每天静脉给予500mg·kgDMTU,连续5d.最后一-1group(D).InHgroup,hyperoxicsolution10ml·kgwasin2次预处理结束后24h,夹闭所有动物腹主动脉肾下段20min,-1-1fusedintravenouslyatarateof30ml·

8、kg·heverydayfor5制作兔脊髓缺血模型.再灌注后48h,对所有动物神经功能days;Inthesameway,Cgroupwereadministredthesame评分,然后处死动物取脊髓(L527),制作标本行组织病理学观volumenormalsalineinsteadofhyperoxicsolution;D+Hgroup察.结果:H组的神经功能评分和脊髓前角正常神经细胞数weretreate