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1、JournalofGeneralVirology(2002),83,2201±2209.PrintedinGreatBritain........................................................................................................................................................................................................................................
2、...........................................Theputativecapsidproteinofthenewlyidenti®edavianhepatitisEvirussharesantigenicepitopeswiththatofswineandhumanhepatitisEvirusesandchickenbigliverandspleendiseasevirusG.Haqshenas,1F.F.Huang,1M.Fenaux,1D.K.Guenette,1F.W.Pierson,1C.T.Larsen,1H.L.Shivaprasad,
3、2T.E.Toth1andX.J.Meng11CenterforMolecularMedicineandInfectiousDisease,DepartmentofBiomedicalSciencesandPathobiology,CollegeofVeterinaryMedicine,VirginiaPolytechnicInstituteandStateUniversity,1410Price'sForkRd,Blacksburg,VA24061-0342,USA2CaliforniaAnimalHealthandFoodSafetyLaboratorySystem,Schoolof
4、VeterinaryMedicine,UniversityofCalifornia-Davis,2789SouthOrangeAve,Fresno,CA93725,USAWerecentlyidenti®edanovelvirus,designatedavianhepatitisEvirus(avianHEV),fromchickenswithhepatitis±splenomegaly(HS)syndromeintheUSA.WeshowedthatavianHEVisgeneticallyrelatedtoswineandhumanHEVs.Herewereporttheantige
5、niccross-reactivityoftheputativeopenreadingframe2(ORF2)capsidproteinofavianHEVwiththoseofswineandhumanHEVsandtheAustralianchickenbigliverandspleendiseasevirus(BLSV).TheregionencodingtheC-terminal268aminoacidresiduesofavianHEVORF2wasclonedintoexpressionvectorpRSET-C.ThetruncatedORF2proteinwasexpre
6、ssedinE.coliasafusionproteinandpuri®edbyaf®nitychromatography.WesternblotanalysisrevealedthattheavianHEVORF2proteinreactedwithantiseraagainsttheSar-55strainofhumanHEVandwithconvalescentantiseraagainstswineHEVandtheUS2strainofhumanHEV,aswellaswithantiserumagainstBLSV.Convalescentserafromspeci®c-pa
7、thogen-freechickensexperimentallyinfectedwithavianHEValsoreactedwiththerecombinantcapsidproteinsofswineHEVandSar-55humanHEV.AntiseraagainsttheUS2humanHEValsoreactedwithrecombinantORF2proteinsofbothswineHEVandSar-55huma
