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1、第28卷第4期中华中医药学刊Vo.l28No.42010年4月CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEApr.2010地亚麻木酚素对糖尿病小鼠肾脏保护作用及机制方项12131111洪兵,徐海娥,胡文,李莉,徐晓文,徐伟,马绍刚,俞伟男(1.淮安市第二人民医院内分泌科,江苏淮安223002;目2.南京医科大学第二附属医院营养科,江苏南京210011;宽3.江苏省疾病预防控制中心,江苏南京210009)带摘要:目的:观察亚麻木酚素提取物(FLE)对糖尿病小鼠早期肾损伤的影
2、响并探讨其作用机制。方法:雄性ICR小鼠分6组:正常对照组,糖尿病模型对照组,糖尿病模型亚麻木酚素提取物[600mg/(kg#d)、1.2g/(kg#d)、3.6g/(kg#d)]治疗组,糖尿病小鼠盐酸二甲双胍[100mg/(kg#d)]治疗组。连续灌胃8周,实验结束后比较各组体重、肾重、肾重/体重、24h尿白蛋白排泄率、肾组织MDA含量及肾组织SOD、GSH-PX活性,HE染色分析肾小球病理形态学改变,综合评价亚麻木酚素提取物对肾脏的保护作用。结果:与糖尿病模型对照组比较,亚麻木酚素提取物中、高剂量组的肾重、2
3、4h尿白蛋白排泄率显著降低(P<0.05),MDA含量降低(P<0105),亚麻木酚素提取物各剂量组的肾脏组织SOD、GSH-Px活性增加(P<0.05)。结论:亚麻木酚素提取物能减轻糖尿病小鼠肾脏损伤,对肾脏有保护作用,其机制与其减轻肾脏氧化应激有关。关键词:木酚素;糖尿病;肾脏;氧化应激中图分类号:R285.5文献标识码:A文章编号:1673-7717(2010)04-0856-04StudyontheProtectiveEffectofFlaxseedLignanExtractonKidneyinDiabe
4、ticMice12131111HONGBing,XUHa-ie,HUWen,LILi,XUXiao-wen,XUWei,MAShao-gang,YUWe-inan(1.Thedepartmentofendocrinology,theSecondPeoples'HospitalofHuaian,Huaian223002,Jiangsu,China;2.Thedepartmentofnutrition,theSecondAffiliatedHospitalofNanjingMedicalUniversity,Nanj
5、ing210011,Jiangsu,China;3.JiangsuProvincialCenterforDiseaseControlandPrevention,Nanjing210009,Jiangsu,China)Abstrac:tObjective:ToinvestigatetheprotectiveeffectofofFlaxseedLignanExtract(FLE)onkidneyindiaeticmice.Methods:ICRmicewererandomlydividedinto6groups:no
6、rmalmicecontro,lSTZdiabeticmicecontro,lSTZd-iabeticmicefedwithFLEatdoseof[600mg/(kg#d),1.2g/(kg#d),3.6g/(kg#d)],andSTZdiabeticmicefedwithmetformin.Theabovecomponentswereadministedintragastricallyfor8weeks.Thentheirbloodglucose,kidneyweighttobodyweight,24-hour
7、urinaryprotein,thecontentofMDAandtheactivitiesofSOD,GSH-Pxofkidneyweredetected.Glomerularstructureofkidneywereexaminedbylightmicroscopy.Results:Thekidneyweighttobodyweight,24-hoururinaryproteininhigh,middledoseofFLEgroupsweresignificantlylowerthanthoseindiabe
8、ticcontrolgroup(P<0.05).ThecontentofMDAinalldoseofFLEgroupsweresignificantlylowerthanthoseindiabeticcontrolgroup(P<0.05).AndtheactivitiesofSOD,GSH-Pxinhigh,middledoseofFLEgroupsweresignif
