阿萨希毛孢子菌体外氟康唑诱导耐药的方法分析

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1、万方数据第三军医大学硕士学位论文InⅥtroStudyonInductionofResistancetoFluconazoIeiⅡ乃站厅D置pD阳露口s露办村BackgrouⅡdandobjectiVeAbstractmc^D点po，_D，zspp．exsitwidelyinnatllre，comprising50species，ofwhichl6speciescancausehumaninfection．Thenumberofinfectionscausedby乃fc^唧D加，zspp．accountfor10％ofthedisseminated血ngalinfe

2、ctions．乃f幽cl印f)加，z口s砌ffisthecommoncausatiVeagentof乃f幽。印D加九spp．infections，andcanleadtosystemicandfataltrichosporonOsis，especiallyini姗姒mocompromisedandimmIlrIodeficienthosts，suchaspeoplesuff色ringf．romneutropenia，orgaIltransplaIltation，hematologicmalignancies，extensivebumsorAIDSWiththewid

3、espreaduseofa11tiflmgaldmgs，especiallyofazoles，theanti如ngalresistanceofZ口s口Jlliihasbeenincreasin91yreported．ThishasmadeclinicaldoctorsdimculttodealwithtrichosporOnosis，andthedeVelopmentofnewanti如ngalagentsoRenrequirelongperiod．Therefbre，itissignificaIlttoexplorethenuconazoleresistancem

4、echanismsandfind0utnewt盯getsofanti向ngalagents．Theseneedtoestablishastableaz01e—resistantmodelofZ口J口^“ResistantmodelsacquiredbyinductioninVitroo托npresentlowsuccessrateandinstableresistaIlce，soitisimperatiVetoestablishamethodwhichcanacquirestableresistantstrainssuccess如11y．Inthisstudy，we

5、obtainednuconazole—resistantmodelofr口s砌ifwhichwereculturedinmediumscontainingmethylprednisoloneandnuconazole，andevaluatedthesuccessrateaIldstability．Methods1．16clinicalandenviror吼entalstrainsofZ以J砌村weretestedthesusc印tibilityagainstnuconaz01einVitr0byCLSIM27一A3；2．Basedonthesusceptibilit

6、yresults，thenuconazolesensitiVestrainswereinducedresistancetonuconazolebyindiVidualfluconazole，indiVidualmethylprednisolone，andnuconaz01ecombinatedwithmethylprednis010nerespectiVely，comparingwimthecontr01万方数据grOup；第二军医人学硕士学位论文3．InordertoobserVethestabilityofaz01e-resistalltstrains，isol

7、atesfoundtoexhibitFLCresistancewereseriallycultured30timesindmg-freemedium．A佼ereachsubculture，antifungalsusc印tibilitytestingwasperfomled；4．Inordertomeasurethedevelopmentofcross—resistancetootheranti允ngalagents，thestablenuconaz01e—resistalltstminswereperf．ormedMICsassayswithfourantinm