激活shh通路对急性脑缺血大鼠突触再生的影响

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1、AbstractEffectofactivatingtheSHHpathwayonexpressionsynapticregenerationinratswithacutecerebralischemiaPostgraduate：LiuHuayanSupervisor：LouJiyu,WangYuzhouDepartmentofNeurology,TheSecondAffiliatedofZhengzhouUniversityZhengzhou450014，HenanDepartmentofNeurology,TheCentralHospitalofJia

2、ozuo454002，HenanBackgroundand0bjectiveAsthesociMandlifestylechanges，themorbidityofcerebrovasculardiseaseareontheriseeveryyear,andtendtobeyoungeLnotonlyleadtoaheavyburdentothecommunityandfamilies，butalsothepsychologicalburdentopatients．Ischemicisthemostcomnlontypeofthecerebrovascul

3、ardisease，whichfeaturesinitshighmorbidity,mortalityandteratogenicrate．Withregardtothecountermeasure，earlydiagnosisandaggressivetreatment，rehabilitationexercisestostrengthenandpreventrecurrenceareessential．Withthecontinuousresearchofneuralprosthetics，nerveregeneration，especiallysyn

4、apticregeneration，isofgreatsignificancetotheischemiccerebrovasculardisease．Recentstudiesindicate，Shhsignaltransductionpathwayandischemiccerebrovasculardiseasemostlikelyhassignificantcorrelationsonthesynapticregenerationperformance．Shhsignalpathincludesthreeprincipalparts-asignalpe

5、ptideShh，thefilmfactorPtch、SmoanddownstreamtranscriptionfactorGli，thecascadeactsorthemutationsoffactorscanactivatethispathway,andeffectsonIVAbstractdownstreamtargetgenesthroughtheGlifinally．ActivationofShhsignalingpathwayhasaprotectiveeffectoncerebralischemia，Callpromotetheregener

6、ationandreconstructionofsynapsesandencouragetherestorationofneurologicalfunction．Thence，thepresentstudytakemethodofLongatomakepermanentischemiamodelinrats，toobservetheexpressionofGlil—downstreammolecularofSonicHedgehogsignalingpathwayandSYN，GAP一43-一synapticassociatedsynthesisprote

7、insinischemicpenumbraatdifferenttimepointsafterapplywiththespecificactivatorPurmorphamine，thus，tofurtherexploretheeffectsandmechanismofShhsignalingpathwayinfluencedonthesynapticregenerationoftheischemiccodex．MaterialsandMethodsPermanentmiddlecerebralareteryocclusionmodelswereprodu

8、cedbysutureembolitechniqueinrats．