醛糖还原酶基因多态性及其活性与2型糖尿病微血管病变的.pdf

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1、Interrelationshipamongthepolymorphismof(AC)ninthei;，-endoftheARgene，theerythrocytealdosereductaseactivityandsusceptibilityofmicroangiopathyinthepatientswithtype2diabetesmellitusAbstractObjectiveTostudytheinterrelationshipamongthepolymorphismof(AC)ninthe5'-endoftheA

2、Rgene,theerythrocytealdosereductaseactivityandsi!sceptibilityofmicroangiopathyinthepatientswithtype2diabetesmellitus(DM).MethodsOnehundredandfortycasesoftype2DMandforty-ninecontrolswereincludedinthisstudy.AllthesecasesweretheHannationalityofGuangxizhuangautonomousr

3、egion.ThesubjectsweredevidedintoDMwithoutdiabeticmicroangiopathy(NDC)(56cases),DMAPgrouy(84cases),andnormalcontrols(CON)(44cases),furthermore,accordingtothetypeofallele,thesubjectsweredevidedintoZ+2,Z-2,bothZ+2andZ-2,neitherZ+2norZ-2(X/X).PCRwasperformedandPCRprodu

4、ctswereelectrophoresedwith8%formamid/ureagel.ARactivity(ARA)wasmeasuredbymodifiedSriratavamethod.ThedifferencesofARAwerecomparedamongthesegroups.ResultsItshowed7typesofalleles(Z-6-Z+6).ThefrequencyofalleleZ+2wasmuchlowerinDMgroupthaninNDCgroupandCONgroup.ARAinDMAPg

5、roupwassignificantlyhigherthanthatinNDCgroup,ARAinNDCgroupwassignificantlyhigherthanthatinCONgroup.AmongZ-2,Z+2,Z+2/Z-2andX/Xgroups.ARAwasthehighestinDMZ-2/Z-2groupandthelowestinDMZ十21Z+2group.ConclusionZ-2.1。allelemightbethesusceptiblegeneandZ+2allelemightbethepro

6、tectedgene.TheactivationofARplaysanimportantroleinthedevelopmentofDMAEZ-2allelemaybeanactivatorandallelemaybeaninhititorofARIKeywords]aldosereductase;polymorphism;type2diabetesmellitus;diateticcomplication;-2-醛糖还原酶基因多态性及其活性与2型糖尿病微血管病变的相关性研究摘要目的研究醛糖还原酶(AR)基因5’端(AC)n

7、的多态性及其活性与2型糖尿病(DM)微血管病变的关系。方法采用聚合酶链反应(PCR)，检测140例2型糖尿病患者，其中无微血管病变组(NDC)56例和微血管病变组(DMAP)94例(糖尿病肾病组(DN)30例，糖尿病视网膜病变组(DRP)24例，糖尿病神经病变组(DPN)30例)和49例正常对照组(CON),AR基因5‘端(AC)n的多态性，同时用改良Sriratava法测定AR活性并比较其在各组间的差异。结果①DM组和CON组均发现7种等位基因(Z-6^-Z+6),Z+2等位基因频率在PKAP组显著低于NDC和CON组(P

8、<0.05).②DMAP组、NDC组和CON三组间的AR活性(ARA)差异有显著性，DMAP组最高，NDC组次之，CON组最低(P<0.001).DMAP组携带Z-2和Z+2等位基因各亚组中，DNZ-2/Z-2组ARA最高，CONZ+2/Z+2组最低，差异有统计学意义(P