腺病毒介导的pdgf-bb基因对大鼠大脑中动脉闭塞模型的作用

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1、四川医学2010年8月第31卷(第8期)SichuanMedicalJournal，2010，Vo1．31，No．8·1053·●基础与实验研究●论著腺病毒介导的PDGF-BB基因对大鼠大脑中动脉闭塞模型的作用宋桂芹(JII~t,医学院医学生物学教研室，四川南充637007)【摘要】目的研究腺病毒介导的PDGF—BB的基因治疗对大鼠大脑中动脉闭塞(MCAO)模型的治疗作用及脑血管发生的影响。方法采用改良的ZeaLonga法制备大鼠脑缺血模型，将30只sD大鼠随机分为假手术组，对照组(IacZ组)和治疗组(Ad·PDGF-BB组)，于造

2、模成功后24h尾静脉给药。检测实验各组神经功能评分恢复情况及脑组织含水率的变化，通过核磁共振和免疫组化分别检测实验各组大脑梗死体积和血管发生的变化。结果治疗组大鼠神经功能恢复对比对照组有显著改善，脑组织含水率和脑梗死面积明显小于对照组，脑血管密度显著高于对照组。结论腺病毒介导的PDGF-BB基因能够显著减小脑组织含水率和脑梗死体积，促进脑部血管发生，为缺血性脑卒中的基因治疗提供了新的途径。【关键词】大脑中动脉闭塞；PDGF—BB；重组腺病毒载体；基因治疗【中图分类号】Q75【文献标识码】A【文章编号】1004-0501(201o}08

3、．1053-03EfectsofadenovirusmediatedPDGF-BBgeneonthemiddlecerebralarteryocclusioninrats．SONGGui-qin．^r0．1lSichuanMedicalCollege，Nanchong，Sichuan637007，China【Abstract】0bjectiveTostudytheefectofAdenovirusmediatedPDGF—BBgeneonthemiddlecerebralarteryocclu．siveratsmodelandtrea

4、tedinacuteischemiccerebrovasculardisease．MethodsThirtyratswererandomlydividedintothreegroups，i．e．，shamgroup，controlgroup(1acZgroup)anddrugtreatmentgroup(Ad—PDGF-BBgroup)．ThemodelofmiddlecerebralarteryocclusioninratswasestablishedsuccessfullybyZeaLongamethod．Twenty-fourh

5、ourslater，Ad-PDGF-BBWasgiventotheratsinAd—PDGF—BBgroupthroughintravenousinjection．TheAd-lacZWasgiventotheratsofthecontrolgroup．Theneurologicaldeficitscoring，thewatercontent，theratsischemicvolumeandthechangeofmicrovesseldensityinthebrainofmiddlecerebralarteryocclusionrat

6、sweredetected．ResultsInthetreatedgroup，therecoveryofneuralfunctionWasimprovedsignificantly，thewaterratioandinfarctsizeofbrainweredecreasedobviouslyandthemicrovesseldensityincreasedremarkably，comparingwiththecontrolgroup，thereweresignificantimprovementintheneurologicalde

7、ficitscoring，thewaterratioandtheratsischemievolumeofthedragtreatmentgroupandthemicrovesseldensityincreasedremarkably．CondusionTheAdenovi—rusmediatedPDGF-BBgenehasobviousefectonthedecreasingthewaterratioandtheratsischemievolume，andpromotingangiogenesis．Thesefindingsmaypr

8、ovideanewstrategyintreatingacuteisehemiccerebrovasculardiseasewithgenetherapy．【Keywords】MCAO；PDGF—BB；recombina