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1、低氧环境、雌二醇及睾酮对红细胞的生成调节的影响【摘要】目的：观察低氧环境、雌二醇及睾酮对家兔红细胞的生成调节的影响。方法：选择健康的成年雄性与雌性家兔各16只分别分成四组，每组4只，分别为试验Ⅰ、Ⅱ、Ⅲ、Ⅳ。对试验Ⅰ组不给予任何处理，作为对照组；试验Ⅱ组用纱布分别捂住家兔的鼻腔与口腔持续30秒，制造低氧环境；试验Ⅲ组分别为家兔注射雌二醇1mg/kg；试验Ⅳ组分别为家兔注射丙酸睾酮1mg/kg。对每一组的家兔进行显微镜记数法测定红细胞数。结果表明：在正常情况下雄性家兔的红细胞数高于雌性家兔；在雄性家兔中，低氧刺激、注射丙酸睾酮过后，红细胞数分别
2、增加了48.54%和53.89%，差异极显著（P<0.01），注射雌二醇后，红细胞数略有减少，差异不显著（P>0.05）；在雌性家兔中，低氧刺激、注射丙酸睾酮过后，红细胞数目分别增加了57.73%和53%，差异极显著（P<0.01），注射雌二醇后，红细胞略有减少，差异不显著（P>0.05）。结论：低氧刺激和注射丙酸睾酮能促进家兔红细胞生成，注射雌二醇能抑制家兔红细胞生成。【关键词】红细胞；低氧环境；雌二醇；睾酮；生成调节TheInfluenceofProductionRegulationonRedBloodCellsundertheHypox
3、iaEnvironment,EstradiolandTestosteroneCollegeofVeterinaryMedicalof2005HuangshiyuinstructorHutingxiuAbstract:Purchasedgrown-upmaleandfemaledomesticrabbits16eachinSichuanagriculturalmarket,observedtwoweeks,allrabbitswerehealthy,thenwestartedexperiments.Dividedthe16maleand16fe
4、malerabbitsintofourgroups,separately,4foreachgroup,namedgroupⅠ,Ⅱ,ⅢandⅣ.InthegroupⅠ,wedidn’tgiveanydisposition,astheblankcontrolgroup;InthegroupⅡ,wecoveredrabbits’nasalcavitiesandtheoralcavitywiththegauzesfor30seconds,tomakehypoxiaenvironment;InthegroupⅢ,weinjected1mgofestra
5、diolintoeachrabbit,respectively;InthegroupⅣ,weinjected1mgofpropionicacidtestosteroneintoeachrabbit,respectively.Thenumberofredbloodcellsofeachgroupweredeterminedbytraditionalmicroscopiccountingwithcountingplates.Theresultsshowedthatthenumberofredbloodcellsinmalerabbitswashi
6、gherthanthefemalerabbits.Inthemalerabbits,certainincreaseinredbloodcellsproductionwerecausedinthehypoxiaenvironmentgroup(groupⅡ)andintheinjectedpropionicacidtestosteronegroup(groupⅣ),theincreaseratewas48.54%and53.89%,respectively,thedifferencewasextremelyremarkable(P<0.01).
7、Inthefemalerabbits,certainincreaseinredbloodcellsproductionwerecausedinthehypoxiaenvironmentgroup(groupⅡ)andintheinjectedpropionicacidtestosteronegroup(groupⅣ),theincreaseratewas57.734%and53%,respectively,thedifferencewasextremelysignificant(P<0.01).Afterinjectingestradiol,
8、thenumberofredbloodcellswasreducedslightly,butthedifferencewasnotsignificant(P>0.0
